Sean Crosson
An Integrative Analysis of Bacterial Signalling Systems
Research Summary
Cells have the extraordinary ability to rapidly modulate their
physiology in response to changes in their environment. This plasticity
is particularly evident in microbial species, many of which adapt to
grow across an extremely diverse range of conditions. Our interests
center on how chemical and physical signals are received, processed,
and integrated by a bacterial cell to generate an adaptive response. To
address these questions, we are using an interdisciplinary set of tools
including NMR and crystallography to explore the structural basis of
signal detection and transduction by sensor histidine kinases, genetics
and array-based transcriptional profiling to decipher the function and
topology of microbial signaling networks, and mathematical modeling to
test our experimentally-derived network topologies.
Selected Papers
Crosson
S and Moffat K. (2001). Structure of a flavin-binding plant
photoreceptor domain: insights into light-mediated signal transduction.
Proc. Natl. Acad. Sci. USA 98: 2995-3000.
Crosson
S and Moffat K. (2002). Photoexcited structure of a plant
photoreceptor domain reveals a light-driven molecular switch. Plant
Cell
14: 1067-1075.
Kennis
J, Crosson S, Gauden M, van Stokkum I, Moffat K and van
Grondelle R. (2003). Primary reactions of the LOV2 domain of
phototropin, a plant blue-light photoreceptor. Biochemistry 42:
3385-3392.
Crosson
S, Rajagopal S and Moffat K. (2003). The LOV domain family:
photoresponsive signaling modules coupled to diverse output domains.
Biochemistry 42: 2-10.
Kennis
J, van Stokkum I, Crosson S, Gauden M, Moffat K and van
Grondelle R. (2004). The LOV2 domain of phototropin: a reversible
photochromic switch. J. Am. Chem. Soc. 126: 4512-4513.
Crosson S, McAdams H and Shapiro L. (2004). A genetic
oscillator and the regulation of cell cycle progression in Caulobacter
crescentus. Cell Cycle 3: 1252-1254.
Crosson S. LOV domain structure, dynamics and diversity.
(2005). In Handbook of Photosensory Receptors ed. W. Briggs and J.
Spudich. Wiley-VCH Verlag. Weinheim, Germany.
Crosson S, McGrath PT, Stephens C, McAdams HH and
Shapiro
L. (2005). Conserved Modular Design of an Oxygen Sensory/Signaling
Network with Species Specific Output. Proc. Natl. Acad. Sci. USA 102:
8018-8023.
Purcell EB, Boutte CC, and Crosson S. (2007).
Two-component networks and cell cycle control in Caulobacter
crescentus. In Bacterial Signal Transduction: Networks and Drug
Targets; ed. R. Utsumi. Landes-Eurekah. Austin, Texas.
Kennis JTM, and Crosson S. (2007). A Bacterial Pathogen
Sees the Light. Science 317:1041-1042.
Purcell EB, Siegal-Gaskins D, Rawling DC, Fiebig A, and
Crosson S. (2007). A Photosensory Two-Component System Regulates
Bacterial Cell Attachment. Proc. Natl. Acad. Sci. USA. 104:18241-18246.
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