Stephen Kron, M.D., Ph.D.

Molecular, Chemical and Systems Biology of Cell Signaling, Cell Cycle and Checkpoint Response

Research Summary

The Kron laboratory is a highly collaborative group of cell biologists, geneticists, biochemists, chemists and computer scientists. Our major basic research and technology efforts are directed at 1) defining roles for chromatin modifications and cell cycle regulators in DNA damage checkpoint response and 2) developing novel mass spectrometry approaches for quantitative phosphoproteomics. We also pursue translational projects directed at 1) developing assays for leukemia diagnostics and therapeutic monitoring and 2) discovering inhibitors of cellular response to DNA double strand breaks as an approach to radiosensitization.

Selected Papers

E. Labay, E.V. Efimova, B.K. Quarshie, D.W. Golden, R.R. Weichselbaum, and S.J. Kron, "Ionizing Radiation Induced Foci (IRIF) persistence screen to discover enhancers of accelerated senescence," Int. J. High Throughput Screening, 2011(2):1-13, 201. (Dovepress)

O.A. Ulanovskaya, J. Cui, S.J. Kron and S.A. Kozmin, "A pairwise chemical genetic screen identifies new inhibitors of glucose transport," Chemistry & Biology, 18:222-30, 2011.
(PubMed)

G. Zhou, J.E. Sylvester, D. Wu, D.R. Veach and S.J. Kron, "A magnetic bead-based protein kinase assay with dual detection techniques," Anal. Biochem., 408:5-11, 2011 (PubMed)

D. Rosetto, A.W. Truman, S.J. Kron and J. Côté, "Epigenetic modifications in double-strand break DNA damage signaling and repair," Clin. Cancer Res., 16:4543-52, 2010 (Clin. Cancer Research)

K. Kristjansdottir and S.J. Kron, "Stable-isotope labeling for protein quantitation by mass spectrometry," Current Proteomics, 7:144-155, 2010 (Current Proteomics)

G. Zhou, X. Yan, D. Wu and S.J. Kron, "Photocleavable peptide-conjugated magnetic beads for protein kinase assays by MALDI-TOF MS," Bioconj. Chem., 21:1917-24, 2010 (PubMed)

M.R. Mand, D. Wu, D.R. Veach and S.J. Kron, "Cell treatment and lysis in 96 well filter-bottom plates for screening Bcr-Abl activity and inhibition in whole cell extracts," J. Biomol. Screening, 15:434-40, 2010 (PubMed)

J.E. Sylvester and S.J. Kron, "A bead-based activity screen for small-molecule inhibitors of signal transduction in chronic myelogenous leukemia cells," Mol. Cancer Ther., 9:1469-81, 2010(PubMed)

D. Wu, J.E. Sylvester, L.L. Parker, G. Zhou and S.J. Kron, "Peptide reporters of kinase activity in whole cell lysates," Biopolymers: Peptide Science, 94:475-86, 2010 (PubMed)

E.V. Efimova, H.J. Mauceri, D.W. Golden, E. Labay, V.P. Bindokas, T.E. Darga, C. Chakraborty, J.C.B. Andrade, C. Crawley, H.G. Sutton, S.J. Kron and R.R. Weichselbaum, "Poly(ADP-ribose) polymerase inhibitor induces accelerated senescence in irradiated breast cancer cells and tumors," Cancer Res., 70:6277-82, 2010 (PubMed)

C.C. Nnakwe, M. Altaf, J. Côté and S.J. Kron, "Dissection of Rad9 BRCT domain function in the mitotic checkpoint response to telomere uncapping," DNA Repair, 8(12):1452-61, 2009(PubMed)

Tobe BT, Kitazono AA, Garcia JS, Gerber RA, Bevis BJ, Choy JS, Chasman D, Kron SJ. (2009) "Morphogenesis signaling components influence cell cycle regulation by cyclin dependent kinase." Cell Div., 4:12. (PubMed)

S.L. Volchenboum, K. Kristjansdottir, D. Wolfgeher, and S.J. Kron, "Rapid validation of Mascot search results via stable isotope labeling, pair picking, and deconvolution of fragmentation patterns," Mol. Cell. Proteomics, 8:2011-22, 2009 (PubMed)

Kristjansdottir K, Wolfgeher D, Lucius N, Angulo DS, Kron SJ. (2008). "Phosphoprotein profiling by PA-GeLC-MS/MS," J. Proteome Research, 7:2812-2824. (PubMed)

Ulanovskaya OA, Janjic J, Suzuki M, Sabharwal SS, Schumacker PT, Kron SJ, Kozmin SA. (2008). "Chemical synthesis enables identification of cytochrome bc1 complex as the cellular target of Leucascandrolide A and Neopeltolide," Nature Chem. Biol., 4:418-24. (PubMed)

Wu, D., Mand, M.R., Veach, D.R., Parker, L.L., Clarkson, B. and Kron, S.J. (2008). "A solid-phase Bcr-Abl kinase assay in 96-well hydrogel plates." Anal Biochem 375:18-26. (PubMed)

Bickenbach, K.A., Veerapong, J., Shao, M.Y., Mauceri, H.J., Posner, M.C., Kron, S.J. and Weichselbaum, R.R. (2008). "Resveratrol is an effective inducer of CArG-driven TNF-alpha gene therapy." Cancer Gene Ther 15:133-9. (PubMed)

Parker, L., Engel-Hall, A., Drew, K., Steinhardt, G., Helseth, Jr. D.L., Jabon, D., McMurry, T., Angulo, D.S. and Kron, S.J. (2008). "Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometry." J Mass Spectrom 43:518-527. (PubMed)